Tosso_apps_development and charract of micosatellite_PR2015

Demenou B.B., Migliore J., Tosso F., Kaymak E. , Hardy O.J.

[2015] Development and characterization of microsatellite markers in the african deciduous tree terminalia superba (Combretaceae). Applications in Plant Sciences 2015 3( 12 ): 1500070 doi:10.3732/apps.1500070

Premise of the study:
Microsatellites were designed and characterized in the African timber forest tree Terminalia superba (Combretaceae). Due to their high variability, these markers are suitable to investigate gene fl ow patterns and the structure of genetic diversity.
Methods and Results:
From a genomic library obtained by next-generation sequencing, seven monomorphic and 14 polymorphic microsatellite loci were developed. The polymorphic microsatellites displayed two to 27 alleles (mean 11.4; expected heterozygosity range 0.283–0.940, mean 0.736) in one population from southeastern Cameroon. Genotypes were typical of an outbreeding diploid species, although null alleles explain a signifi cant heterozygote defi cit in three loci. Cross-amplifi cation in three congeneric species ( T. ivorensis , T. avicennioides , and T. mantaly ) failed, suggesting that T. superba is rather divergent.
This set of newly developed microsatellite markers will be useful for assessing the genetic diversity, population structure, and demographic history of T. superba in tropical African forests.

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